|Other Names||Delta(24)-sterol reductase, 24-dehydrocholesterol reductase, 3-beta-hydroxysterol delta-24-reductase, Diminuto/dwarf1 homolog, Seladin-1, DHCR24, KIAA0018|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2840c was selected from the Center region of human DHCR24. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the reduction of the delta-24 double bond of sterol intermediates. Protects cells from oxidative stress by reducing caspase 3 activity during apoptosis induced by oxidative stress. Also protects against amyloid-beta peptide-induced apoptosis.|
|Cellular Location||Endoplasmic reticulum membrane; Single-pass membrane protein. Golgi apparatus membrane; Single-pass membrane protein|
|Tissue Location||Highly expressed in brain and adrenal gland with moderate expression in liver, lung, spleen, prostate and spinal cord. Low expression in heart, uterus and prostate Undetectable in blood cells. In the brain, strongly expressed in cortical regions, substantia nigra, caudate nucleus, hippocampus, medulla oblongata and pons. In brains affected by Alzheimer disease, expression in the inferior temporal lobe is substantially lower than in the frontal cortex.|
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Provided below are standard protocols that you may find useful for product applications.
DHCR24 is a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. This protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in DHCR24 gene have been associated with desmosterolosis. Also, reduced expression of DHCR24 gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells.
Greeve I., Hermans-Borgmeyer I.J. Neurosci. 20:7345-7352(2000)Waterham H.R., Koster J.Am. J. Hum. Genet. 69:685-694(2001)
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