|Other Names||DNA polymerase delta catalytic subunit, DNA polymerase subunit delta p125, POLD1, POLD|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2867a was selected from the N-term region of human POLD1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.|
|Tissue Location||Expressed across a panel of tissues, with high levels of expression in heart and lung|
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Provided below are standard protocols that you may find useful for product applications.
The DNA polymerase delta complex is involved in DNA replication and repair, and it consists of the proliferating cell nuclear antigen (PCNA), the multisubunit replication factor C , and the 4 subunit polymerase complex: POLD1, POLD2 , POLD3 , and POLD4.
Chung D.W., Zhang J.,Proc. Natl. Acad. Sci. U.S.A. 88:11197-11201(1991)Yang C.-L., Chang L.-S.Nucleic Acids Res. 20:735-745(1992) Li H., Xie B., Zhou Y., Rahmeh A.J. Biol. Chem. 281:14748-14755(2006)
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