|Other Names||Exonuclease 1, hExo1, 31--, Exonuclease I, hExoI, EXO1, EXOI, HEX1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP2871a was selected from the N-term region of human EXO1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch- containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.|
|Cellular Location||Nucleus Note=Colocalizes with PCNA to discrete nuclear foci in S-phase|
|Tissue Location||Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach|
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Provided below are standard protocols that you may find useful for product applications.
EXO1 is a protein with 5' to 3' exonuclease activity as well as an RNase H activity. This protein is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination.
Tishkoff D.X., Amin N.S., Viars C.S.Cancer Res. 58:5027-5031(1998)Lee B.-I., Nguyen L.H.,Nucleic Acids Res. 30:942-949(2002)Alam N.A., Gorman P., Jaeger E.E.M.Cancer Genet. Cytogenet. 147:121-127(2003)Knudsen N.O., Nielsen F.C., Vinther L.Nucleic Acids Res. 35:2609-2619(2007)
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