|Other Names||NF-kappa-B essential modulator, NEMO, FIP-3, IkB kinase-associated protein 1, IKKAP1, Inhibitor of nuclear factor kappa-B kinase subunit gamma, I-kappa-B kinase subunit gamma, IKK-gamma, IKKG, IkB kinase subunit gamma, NF-kappa-B essential modifier, IKBKG, FIP3, NEMO|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP3130a was selected from the region of human Phospho-IKKg-S43. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Its binding to scaffolding polyubiquitin seems to play a role in IKK activation by multiple signaling receptor pathways. However, the specific type of polyubiquitin recognized upon cell stimulation (either 'Lys-63'- linked or linear polyubiquitin) and its functional importance is reported conflictingly. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B- mediated protection from cytokine toxicity. Essential for viral activation of IRF3. Involved in TLR3- and IFIH1-mediated antiviral innate response; this function requires 'Lys-27'-linked polyubiquitination.|
|Cellular Location||Cytoplasm. Nucleus. Note=Sumoylated NEMO accumulates in the nucleus in response to genotoxic stress|
|Tissue Location||Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas|
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Provided below are standard protocols that you may find useful for product applications.
IKKg is a regulatory subunit part of the IKK-signalosome complex activation. It is also considered to be a mediator for TAX activation of NF-kappa-B. Additionally, this protein could be implicated in NF-kappa-B-mediated protection from cytokine toxicity. Mutations in IKKg are the cause of anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID) and of familial incontinentia pigmenti type II (IP2).
Reiley, W., et al., Mol. Cell. Biol. 25(10):3886-3895 (2005).Wilson, G.M., et al., Mol. Biol. Cell 16(2):849-860 (2005).Nishikomori, R., et al., Blood 103(12):4565-4572 (2004).Orange, J.S., et al., J. Allergy Clin. Immunol. 114(3):650-656 (2004).Saito, K., et al., Structure (Camb.) 12(9):1719-1728 (2004).
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