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Phospho-SPRY4(Y75) Antibody Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9C004 |
|---|---|
| Clone Names | 80130153 |
| Gene ID | 81848 |
|---|---|
| Other Names | Protein sprouty homolog 4, Spry-4, SPRY4 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP3585a was selected from the region of human Phospho-SPRY4-pY75. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | SPRY4 |
|---|---|
| Function | Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras (PubMed:12027893). Probably impairs the formation of GTP-Ras (PubMed:12027893). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (PubMed:12717443). Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed:15584898). |
| Cellular Location | Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor) (By similarity). Colocalizes with TESK1 in vesicular spots in the cytoplasm (PubMed:15584898) {ECO:0000250|UniProtKB:Q9WTP2, ECO:0000269|PubMed:15584898} |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.
References
Tsai CF, et al. (2008) J Proteome ResGuo A, et al. (2008) Proc Natl Acad Sci U S A 105, 692-7Rikova K, et al. (2007) Cell 131, 1190-203Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM (2007) Proc Natl Acad Sci U S A 104, 5860-5Tsumura Y, et al. (2005) Biochem J 387, 627-37
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