Phospho-IPF(T11) Antibody Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P52945 |
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Clone Names | 80130213 |
Gene ID | 3651 |
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Other Names | Pancreas/duodenum homeobox protein 1, PDX-1, Glucose-sensitive factor, GSF, Insulin promoter factor 1, IPF-1, Insulin upstream factor 1, IUF-1, Islet/duodenum homeobox-1, IDX-1, Somatostatin-transactivating factor 1, STF-1, PDX1, IPF1, STF1 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP3617a was selected from the T11 region of human Phospho-IPF. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | PDX1 |
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Synonyms | IPF1, STF1 |
Function | Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. |
Cellular Location | Nucleus. Cytoplasm, cytosol. |
Tissue Location | Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells) |
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Provided below are standard protocols that you may find useful for product applications.
Background
PDX1 is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in its gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4).
References
Lebrun P, Montminy MR, Van Obberghen E (2005) J Biol Chem 280, 38203-10
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