|Other Names||Mitogen-activated protein kinase 8, MAP kinase 8, MAPK 8, JNK-46, Stress-activated protein kinase 1c, SAPK1c, Stress-activated protein kinase JNK1, c-Jun N-terminal kinase 1, MAPK8, JNK1, PRKM8, SAPK1, SAPK1C|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||JNK1, PRKM8, SAPK1, SAPK1C|
|Function||Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH. Phosphorylates the CLOCK-ARNTL/BMAL1 heterodimer and plays a role in the regulation of the circadian clock (PubMed:22441692).|
|Cellular Location||Cytoplasm. Nucleus|
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The protein encoded by this gene is a member of the MAPkinase family. MAP kinases act as an integration point for multiplebiochemical signals, and are involved in a wide variety of cellularprocesses such as proliferation, differentiation, transcriptionregulation and development. This kinase is activated by variouscell stimuli, and targets specific transcription factors, and thusmediates immediate-early gene expression in response to cellstimuli. The activation of this kinase by tumor-necrosis factoralpha (TNF-alpha) is found to be required for TNF-alpha inducedapoptosis. This kinase is also involved in UV radiation inducedapoptosis, which is thought to be related to cytochrom c-mediatedcell death pathway. Studies of the mouse counterpart of this genesuggested that this kinase play a key role in T cell proliferation,apoptosis and differentiation. Four alternatively splicedtranscript variants encoding distinct isoforms have been reported.
Dasgupta, J., et al. J. Cell. Physiol. 225(1):52-62(2010)Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)Gao, D., et al. J. Biol. Chem. 285(39):29965-29973(2010)Han, J.S., et al. Anticancer Res. 30(9):3407-3412(2010)Yang, X.H., et al. World J. Gastroenterol. 16(27):3385-3393(2010)
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