|Other Names||Hyaluronan synthase 2, Hyaluronate synthase 2, Hyaluronic acid synthase 2, HA synthase 2, HAS2|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction and it is particularly responsible for the synthesis of high molecular mass hyaluronan. Required for the transition of endocardial cushion cells into mesenchymal cells, a process crucial for heart development. May also play a role in vasculogenesis. High molecular mass hyaluronan also play a role in early contact inhibition a process which stops cell growth when cells come into contact with each other or the extracellular matrix (By similarity).|
|Cellular Location||Membrane; Multi-pass membrane protein|
|Tissue Location||Expressed in fibroblasts.|
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Hyaluronan or hyaluronic acid (HA) is a high molecularweight unbranched polysaccharide synthesized by a wide variety oforganisms from bacteria to mammals, and is a constituent of theextracellular matrix. It consists of alternating glucuronic acidand N-acetylglucosamine residues that are linked by beta-1-3 andbeta-1-4 glycosidic bonds. HA is synthesized by membrane-boundsynthase at the inner surface of the plasma membrane, and thechains are extruded through pore-like structures into theextracellular space. It serves a variety of functions, includingspace filling, lubrication of joints, and provision of a matrixthrough which cells can migrate. HA is actively produced duringwound healing and tissue repair to provide a framework for ingrowthof blood vessels and fibroblasts. Changes in the serumconcentration of HA are associated with inflammatory anddegenerative arthropathies such as rheumatoid arthritis. Inaddition, the interaction of HA with the leukocyte receptor CD44 isimportant in tissue-specific homing by leukocytes, andoverexpression of HA receptors has been correlated with tumormetastasis. HAS2 is a member of the newly identified vertebrategene family encoding putative hyaluronan synthases, and its aminoacid sequence shows significant homology to glycosaminoglycansynthetase (DG42) from Xenopus laevis, and human and murinehyaluronan synthase 1.
Simpson, M.A., et al. J. Biol. Chem. 277(12):10050-10057(2002)Spicer, A.P., et al. Biochem. Soc. Trans. 27(2):109-115(1999)Spicer, A.P., et al. Genomics 41(3):493-497(1997)Watanabe, K., et al. J. Biol. Chem. 271(38):22945-22948(1996)
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