|Other Names||Chromatin assembly factor 1 subunit A, CAF-1 subunit A, Chromatin assembly factor I p150 subunit, CAF-I 150 kDa subunit, CAF-I p150, hp150, CHAF1A, CAF, CAF1P150|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity).|
|Cellular Location||Nucleus. Note=DNA replication foci|
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Provided below are standard protocols that you may find useful for product applications.
Chromatin assembly factor I (CAF1) is a nuclear complexconsisting of p50, p60 (CHAF1B; MIM 601245), and p150 (CHAF1A)subunits that assembles histone octamers onto replicating DNA invitro (Kaufman et al., 1995 [PubMed 7600578]).
Murzina, N., et al. Mol. Cell 4(4):529-540(1999)Shibahara, K., et al. Cell 96(4):575-585(1999)Marheineke, K., et al. J. Biol. Chem. 273(24):15279-15286(1998)Verreault, A., et al. Cell 87(1):95-104(1996)
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