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P-Glycoprotein (ABCB1) Antibody (Center) Blocking peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | P08183 |
|---|---|
| Clone Names | 2092001 |
| Gene ID | 5243 |
|---|---|
| Other Names | Multidrug resistance protein 1, ATP-binding cassette sub-family B member 1, P-glycoprotein 1, CD243, ABCB1, MDR1, PGY1 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP6111a was selected from the Center region of human ABCB1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | ABCB1 (HGNC:40) |
|---|---|
| Synonyms | MDR1, PGY1 |
| Function | Translocates drugs and phospholipids across the membrane (PubMed:8898203, PubMed:2897240, PubMed:9038218, PubMed:35970996). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D- glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug- resistant cells (PubMed:2897240, PubMed:9038218, PubMed:35970996). |
| Cellular Location | Cell membrane; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441} Apical cell membrane. Cytoplasm Note=ABCB1 localization is influenced by C1orf115 expression levels (plasma membrane versus cytoplasm). Localized to the apical membrane of enterocytes (PubMed:28408210). |
| Tissue Location | Expressed in small intestine (PubMed:28408210). Expressed in liver, kidney and brain. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
The membrane-associated ABCB1 protein is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. ABCB1 is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier.
References
Saito, S., et al., J. Hum. Genet. 47(1):38-50 (2002).Kerb, R., et al., Pharmacogenomics 2(1):51-64 (2001).Cascorbi, I., et al., Clin. Pharmacol. Ther. 69(3):169-174 (2001).Hoffmeyer, S., et al., Proc. Natl. Acad. Sci. U.S.A. 97(7):3473-3478 (2000).Mickley, L.A., et al., Blood 91(5):1749-1756 (1998).
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