|Other Names||Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2, Lysophosphatidic acid receptor Edg-4, LPAR2, EDG4, LPA2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6140a was selected from the N-term region of human EDG4 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Seems to be coupled to the G(i)/G(o), G(12)/G(13), and G(q) families of heteromeric G proteins. Plays a key role in phospholipase C-beta (PLC-beta) signaling pathway. Stimulates phospholipase C (PLC) activity in a manner that is independent of RALA activation.|
|Cellular Location||Cell surface. Cell membrane; Multi-pass membrane protein. Note=Prior to LPA treatment found predominantly at the cell surface but in the presence of LPA colocalizes with RALA in the endocytic vesicles|
|Tissue Location||Expressed most abundantly in testes and peripheral blood leukocytes with less expression in pancreas, spleen, thymus and prostate. Little or no expression in heart, brain, placenta, lung, liver, skeletal muscle, kidney, ovary, small intestine, or colon|
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Provided below are standard protocols that you may find useful for product applications.
EDG4 is a member of family I of the G protein-coupled receptors, as well as the EDG family of proteins. This protein functions as a lysophosphatidic acid (LPA) receptor and contributes to Ca2+ mobilization, a critical cellular response to LPA in cells, through association with Gi and Gq proteins.
Hu, Y.L., et al., J. Natl. Cancer Inst. 95(10):733-740 (2003).Fujita, T., et al., Cancer Lett. 192(2):161-169 (2003).Contos, J.J., et al., Genomics 64(2):155-169 (2000).Zheng, Y., et al., FASEB J. 14(15):2387-2389 (2000).Young, K.W., et al., J. Biol. Chem. 275(49):38532-38539 (2000).
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