|Other Names||Macrophage metalloelastase, MME, Macrophage elastase, ME, hME, Matrix metalloproteinase-12, MMP-12, MMP12, HME|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6196a was selected from the C-term region of human MMP12 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
|Tissue Location||Found in alveolar macrophages but not in peripheral blood monocytes|
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Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP12 may be involved in tissue injury and remodeling. This protein has significant elastolytic activity. MMP12 can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, wtih small hydrophobic residues (prefereably alanine) occupying P3. The protein is found in alveolar macrophages but not in peripheral blood monocytes. MMP12 can be induced by exposure to lypopolysaccharide, and is inhibited by dexamethasone.
Nar, H., et al., J. Mol. Biol. 312(4):743-751 (2001).Lang, R., et al., J. Mol. Biol. 312(4):731-742 (2001).Gronski, T.J. Jr., et al., J. Biol. Chem. 272(18):12189-12194 (1997).Shapiro, S.D., et al., J. Biol. Chem. 268(32):23824-23829 (1993).
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