|Other Names||Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, HsCdc18, p62(cdc6), HsCDC6, CDC6, CDC18L|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6271a was selected from the N-term region of human Cdc6. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.|
|Cellular Location||Nucleus. Cytoplasm. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells|
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Provided below are standard protocols that you may find useful for product applications.
Human Cdc6 is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.
Alexandrow, M.G., et al., Mol. Cell. Biol. 24(4):1614-1627 (2004).Yim, H., et al., Mol. Biol. Cell 14(10):4250-4259 (2003).Clay-Farrace, L., et al., EMBO J. 22(3):704-712 (2003).Pelizon, C., et al., EMBO Rep. 3(8):780-784 (2002).Bermejo, R., et al., Mol. Biol. Cell 13(11):3989-4000 (2002).
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