|Other Names||Crossover junction endonuclease EME1, 3122-, MMS4 homolog, hMMS4, EME1, MMS4|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6288a was selected from the N-term region of human EME1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'- flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks.|
|Cellular Location||Nucleus, nucleolus. Note=Recruited to regions of DNA damage in S-phase cells|
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Provided below are standard protocols that you may find useful for product applications.
EME1 and MUS81 (MIM 606591) form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication (Abraham et al., 2003 [PubMed 14609959]).[supplied by OMIM]
Taylor,E.R., Proc. Natl. Acad. Sci. U.S.A. 105 (10), 3757-3762 (2008)Olsen,J.V., Cell 127 (3), 635-648 (2006)
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