|Other Names||Gamma-secretase subunit PEN-2, Presenilin enhancer protein 2, PSENEN, PEN2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6301e was selected from the Center region of human PEN2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:12522139, PubMed:12763021, PubMed:12740439, PubMed:12679784, PubMed:24941111). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable). PSENEN modulates both endoproteolysis of presenilin and gamma-secretase activity (PubMed:12522139, PubMed:12763021, PubMed:12740439, PubMed:12679784, PubMed:24941111).|
|Cellular Location||Endoplasmic reticulum membrane; Multi- pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein Membrane; Multi-pass membrane protein Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi.|
|Tissue Location||Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.|
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Provided below are standard protocols that you may find useful for product applications.
Presenilins, which are components of the gamma-secretase protein complex, are required for intramembranous processing of some type I transmembrane proteins, such as the Notch proteins and the beta-amyloid precursor protein. Signaling by Notch receptors mediates a wide range of developmental cell fates. Processing of the beta-amyloid precursor protein generates neurotoxic amyloid beta peptides, the major component of senile plaques associated with Alzheimer's disease. PEN2 is required for Notch pathway signaling, and for the activity and accumulation of gamma-secretase.
Marlow, L., et al., Biochem. Biophys. Res. Commun. 305(3):502-509 (2003).Crystal, A.S., et al., J. Biol. Chem. 278(22):20117-20123 (2003).Takasugi, N., et al., Nature 422(6930):438-441 (2003).Steiner, H., et al., J. Biol. Chem. 277(42):39062-39065 (2002).Francis, R., et al., Dev. Cell 3(1):85-97 (2002).
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