|Other Names||Gamma-secretase subunit PEN-2, Presenilin enhancer protein 2, PSENEN, PEN2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6301f was selected from the C-term region of human PEN2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein Note=Predominantly located in the endoplasmic reticulum and in the cis-Golgi.|
|Tissue Location||Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.|
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Provided below are standard protocols that you may find useful for product applications.
Presenilins, which are components of the gamma-secretase protein complex, are required for intramembranous processing of some type I transmembrane proteins, such as the Notch proteins and the beta-amyloid precursor protein. Signaling by Notch receptors mediates a wide range of developmental cell fates. Processing of the beta-amyloid precursor protein generates neurotoxic amyloid beta peptides, the major component of senile plaques associatedwith Alzheimer's disease. This gene encodes a protein that is required for Notch pathway signaling, and for the activity and accumulation of gamma-secretase.
1. Marlow, L., et al., Biochem. Biophys. Res. Commun. 305(3):502-509 (2003). 2. Crystal, A.S., et al., J. Biol. Chem. 278(22):20117-20123 (2003). 3. Takasugi, N., et al., Nature 422(6930):438-441 (2003). 4. Steiner, H., et al., J. Biol. Chem. 277(42):39062-39065 (2002). 5. Francis, R., et al., Dev. Cell 3(1):85-97 (2002).
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