|Other Names||Signal peptide peptidase-like 2A, SPP-like 2A, SPPL2a, 3423-, Intramembrane protease 3, IMP-3, Presenilin-like protein 2, SPPL2A, IMP3, PSL2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6312c was selected from the C-term region of human SPPL2a. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in FASLG, ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. Also responsible for the intramembrane cleavage of Fas antigen ligand FASLG, which promotes the release of the intracellular FasL domain (FasL ICD). May play a role in the regulation of innate and adaptive immunity.|
|Cellular Location||Late endosome membrane; Multi- pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Cell membrane. Note=Colocalizes with palmitoylated and myristoylated proteins at the plasma membrane|
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Provided below are standard protocols that you may find useful for product applications.
By searching sequence databases for homologs of Dictyostelium discoideum Impas, which shares homology with presenilin, followed by PCR of lymphocyte and hippocampus cDNA libraries, Grigorenko et al. (2002) cloned SPPL2a. This protein, which may act as an intramembrane protease, contains an N-terminal protease-associated (PA) domain, several transmembrane regions, a hydrophilic loop, conservative sequences around the first and second aspartate residues, and an invariant PAL motif near the C terminus.
Friedmann, E., et al., J. Biol. Chem. 279(49):50790-50798 (2004).Grigorenko, A.P., et al., Biochemistry Mosc. 67(7):826-835 (2002).Weihofen, A., et al., Science 296(5576):2215-2218 (2002).
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