BUB3 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | O43684 |
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Clone Names | 80924085 |
Gene ID | 9184 |
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Other Names | Mitotic checkpoint protein BUB3, BUB3 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP6629b was selected from the C-term region of human BUB3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | BUB3 |
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Function | Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. |
Cellular Location | Nucleus. Chromosome, centromere, kinetochore. Note=Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I- anaphase I (MI-AI) transition. |
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Provided below are standard protocols that you may find useful for product applications.
Background
BUB3 is a protein involved in spindle checkpoint function. The protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein.
References
Vernole,P., Cell Cycle 8 (3), 421-429 (2009)Logarinho,E., Mol. Biol. Cell 19 (4), 1798-1813 (2008)
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