NKRF Antibody (Center) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | O15226 |
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Clone Names | 80917093 |
Gene ID | 55922 |
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Other Names | NF-kappa-B-repressing factor, NFkB-repressing factor, Protein ITBA4, Transcription factor NRF, NKRF, ITBA4, NRF |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP6660c was selected from the Center region of human NKRF. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | NKRF {ECO:0000303|PubMed:32179686, ECO:0000312|HGNC:HGNC:19374} |
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Function | Enhances the ATPase activity of DHX15 by acting like a brace that tethers mobile sections of DHX15 together, stabilizing a functional conformation with high RNA affinity of DHX15 (PubMed:12381793). Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity (PubMed:12381793). Also involved in the regulation of IL-8 transcription (PubMed:12381793). May also act as a DNA-binding transcription regulator: interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes (PubMed:10562553). |
Cellular Location | Nucleus, nucleolus. |
Tissue Location | Widely and constitutively expressed (PubMed:10562553). Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney (PubMed:10562553) |
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Provided below are standard protocols that you may find useful for product applications.
Background
NKRF is a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm.
References
Ho,S.C., J. Immunol. 183 (1), 411-420 (2009)Glanz,A., Immunol. Cell Biol. 86 (7), 622-630 (2008)
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