|Other Names||Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase, PNGase, hPNGase, N-glycanase 1, Peptide:N-glycanase, NGLY1, PNG1|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Specifically deglycosylates the denatured form of N- linked glycoproteins in the cytoplasm and assists their proteasome-mediated degradation. Cleaves the beta-aspartyl- glucosamine (GlcNAc) of the glycan and the amide side chain of Asn, converting Asn to Asp. Prefers proteins containing high- mannose over those bearing complex type oligosaccharides. Can recognize misfolded proteins in the endoplasmic reticulum that are exported to the cytosol to be destroyed and deglycosylate them, while it has no activity toward native proteins. Deglycosylation is a prerequisite for subsequent proteasome-mediated degradation of some, but not all, misfolded glycoproteins.|
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Provided below are standard protocols that you may find useful for product applications.
NGLY1 is an enzyme that catalyzes hydrolysis ofan N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue toN-acetyl-beta-D-glucosaminylamine and a peptide containing anaspartate residue. The encoded enzyme may play a role in theproteasome-mediated degradation of misfolded glycoproteins.
Altrich-VanLith, M.L., et al. J. Immunol. 177(8):5440-5450(2006)Allen, M.D., et al. J. Biol. Chem. 281(35):25502-25508(2006)Katiyar, S., et al. Mol. Biol. Cell 16(10):4584-4594(2005)
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