|Other Names||GTP-binding nuclear protein Ran, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein, RAN, ARA24|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export. Involved in chromatin condensation and control of cell cycle (By similarity). The complex with BIRC5/ survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2.|
|Cellular Location||Nucleus. Cytoplasm. Melanosome. Note=Predominantly nuclear during interphase (By similarity) Becomes dispersed throughout the cytoplasm during mitosis Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
|Tissue Location||Expressed in a variety of tissues.|
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RAN (ras-related nuclear protein) is a small GTP bindingprotein belonging to the RAS superfamily that is essential for thetranslocation of RNA and proteins through the nuclear pore complex.The RAN protein is also involved in control of DNA synthesis andcell cycle progression. Nuclear localization of RAN requires thepresence of regulator of chromosome condensation 1 (RCC1).Mutations in RAN disrupt DNA synthesis. Because of its manyfunctions, it is likely that RAN interacts with several otherproteins. RAN regulates formation and organization of themicrotubule network independently of its role in thenucleus-cytosol exchange of macromolecules. RAN could be a keysignaling molecule regulating microtubule polymerization duringmitosis. RCC1 generates a high local concentration of RAN-GTParound chromatin which, in turn, induces the local nucleation ofmicrotubules. RAN is an androgen receptor (AR) coactivator thatbinds differentially with different lengths of polyglutamine withinthe androgen receptor. Polyglutamine repeat expansion in the AR islinked to Kennedy's disease (X-linked spinal and bulbar muscularatrophy). RAN coactivation of the AR diminishes with polyglutamineexpansion within the AR, and this weak coactivation may lead topartial androgen insensitivity during the development of Kennedy'sdisease.
Kim, J.S., et al. Mol. Carcinog. 49(10):913-921(2010)Liu, C., et al. Mol. Cell. Biol. 30(16):3956-3969(2010)Boni, V., et al. Pharmacogenomics J. (2010) In press :Ly, T.K., et al. J. Biol. Chem. 285(8):5815-5826(2010)Mishra, R.K., et al. Nat. Cell Biol. 12(2):164-169(2010)
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