|Other Names||Mothers against decapentaplegic homolog 7, MAD homolog 7, Mothers against DPP homolog 7, Mothers against decapentaplegic homolog 8, MAD homolog 8, Mothers against DPP homolog 8, SMAD family member 7, SMAD 7, Smad7, hSMAD7, SMAD7, MADH7, MADH8|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6753b was selected from the C-term region of human Smad7. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A- PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.|
|Cellular Location||Nucleus Cytoplasm Note=Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236)|
|Tissue Location||Ubiquitous with higher expression in the lung and vascular endothelium|
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Provided below are standard protocols that you may find useful for product applications.
Smad7 is a member of the MAD-related family of molecules. MAD-related proteins are a recently identified family of intracellular proteins that are thought to be essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta super family.
Hirata,H., et.al., Cancer 115 (19), 4488-4503 (2009)
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