|Other Names||Methylosome protein 50, MEP-50, Androgen receptor cofactor p44, WD repeat-containing protein 77, p44/Mep50, WDR77, MEP50, WD45|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.|
|Cellular Location||Nucleus. Cytoplasm. Note=Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors|
|Tissue Location||Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.|
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Provided below are standard protocols that you may find useful for product applications.
WDR77 is a component of the 20S PRMT5 (MIM604045)-containing methyltransferase complex, which modifiesspecific arginines to dimethylarginines in several spliceosomal Smproteins (see MIM 601061). This modification targets Sm proteins tothe survival of motor neurons (SMN) complex (see MIM 600354) forassembly into small nuclear ribonucleoprotein core particles(Friesen et al., 2002 [PubMed 11756452]).
Vascotto, C., et al. Mol. Cell. Biol. 29(7):1834-1854(2009)Peng, Y., et al. Proc. Natl. Acad. Sci. U.S.A. 105(13):5236-5241(2008)Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)Liang, J.J., et al. J. Urol. 177(5):1918-1922(2007)Lamesch, P., et al. Genomics 89(3):307-315(2007)
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