EIF2B2 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P49770 |
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Clone Names | 80703065 |
Gene ID | 8892 |
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Other Names | Translation initiation factor eIF-2B subunit beta, S20I15, S20III15, eIF-2B GDP-GTP exchange factor subunit beta, EIF2B2, EIF2BB |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP6781a was selected from the N-term region of human EIF2B2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | EIF2B2 |
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Synonyms | EIF2BB |
Function | Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit (PubMed:25858979, PubMed:27023709, PubMed:31048492). Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl- initiator methionine tRNA available to the ribosome and consequently global translation is repressed (PubMed:25858979, PubMed:31048492). |
Cellular Location | Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9UT76} |
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Provided below are standard protocols that you may find useful for product applications.
Background
Eukaryotic initiation factor-2B (EIF2B) is a GTP exchange protein essential for protein synthesis. It consists of alpha (EIF2B1; MIM 606686), beta (EIF2B2), gamma (EIF2B3; MIM 606273), delta (EIF2B4; MIM 606687), and epsilon (EIF2B5; MIM 603945) subunits. EIF2B activates its EIF2 (see MIM 603907) substrate by exchanging EIF2-bound GDP for GTP.
References
Pronk,J., et.al., Mult. Scler. 14 (8), 1123-1126 (2008)Llorens,F.,et.al., Biochem. J. 394 (PT 1), 227-236 (2006)
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