|Other Names||Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, Short-chain enoyl-CoA hydratase, SCEH, ECHS1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP6907b was selected from the C-term region of human ECHS1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Straight-chain enoyl-CoA thioesters from C4 up to at least C16 are processed, although with decreasing catalytic rate. Has high substrate specificity for crotonyl-CoA and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA and methacrylyl-CoA. It is noteworthy that binds tiglyl-CoA, but hydrates only a small amount of this substrate.|
|Cellular Location||Mitochondrion matrix.|
|Tissue Location||Liver, fibroblast, muscle. Barely detectable in spleen and kidney|
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Provided below are standard protocols that you may find useful for product applications.
ECHS1 functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. It is a member of the hydratase/isomerase superfamily.
Bruneel,A., et.al., Proteomics 5 (15), 3876-3884 (2005)
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