ECHS1 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P30084 |
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Clone Names | 90415036 |
Gene ID | 1892 |
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Other Names | Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, Short-chain enoyl-CoA hydratase, SCEH, ECHS1 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP6907b was selected from the C-term region of human ECHS1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | ECHS1 (HGNC:3151) |
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Function | Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl- CoA) to the corresponding (3S)-3hydroxyacyl-CoA species through addition of a water molecule to the double bond (PubMed:25125611, PubMed:26251176). Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16 (PubMed:26251176). Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3- methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA) (PubMed:26251176). Can bind tiglyl-CoA (2- methylcrotonoyl-CoA), but hydrates only a small amount of this substrate (PubMed:26251176). Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation (PubMed:25125611, PubMed:26251176). At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)- hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl- CoA species (such as (3S)-hydroxyhexanoyl-CoA) (By similarity). |
Cellular Location | Mitochondrion matrix. |
Tissue Location | Liver, fibroblast, muscle. Barely detectable in spleen and kidney |
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Provided below are standard protocols that you may find useful for product applications.
Background
ECHS1 functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. It is a member of the hydratase/isomerase superfamily.
References
Bruneel,A., et.al., Proteomics 5 (15), 3876-3884 (2005)
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