|Other Names||Serine/threonine-protein kinase D3, Protein kinase C nu type, Protein kinase EPK2, nPKC-nu, PRKD3, EPK2, PRKCN|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7025b was selected from the C-term region of human PKC nu . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress (By similarity).|
|Cellular Location||Cytoplasm. Membrane. Note=Translocation to the cell membrane is required for kinase activation|
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Provided below are standard protocols that you may find useful for product applications.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC nu is one of the PKC family members. This kinase can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. This kinase can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phopholipase C gamma and the involvement of other PKC family members.
Yeaman, C., et al., Nat. Cell Biol. 6(2):106-112 (2004).Rey, O., et al., J. Biol. Chem. 278(26):23773-23785 (2003).Matthews, S.A., et al., J. Biol. Chem. 278(11):9086-9091 (2003).Bennasser, Y., et al., Virology 303(1):174-180 (2002).Bennasser, Y., et al., FASEB J. 16(6):546-554 (2002).
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