FN3X Antibody (Center) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9HA64 |
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Clone Names | 4011928 |
Gene ID | 79672 |
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Other Names | Ketosamine-3-kinase, 271-, Fructosamine-3-kinase-related protein, FN3K-RP, FN3K-related protein, FN3KRP |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7067a was selected from the Center region of human FN3X. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | FN3KRP {ECO:0000303|PubMed:15137908, ECO:0000312|HGNC:HGNC:25700} |
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Function | Ketosamine-3-kinase involved in protein deglycation by mediating phosphorylation of ribuloselysine and psicoselysine on glycated proteins, to generate ribuloselysine-3 phosphate and psicoselysine-3 phosphate, respectively (PubMed:14633848, PubMed:15137908). Ribuloselysine-3 phosphate and psicoselysine-3 phosphate adducts are unstable and decompose under physiological conditions (PubMed:14633848, PubMed:15137908). Not able to phosphorylate fructoselysine (PubMed:14633848). |
Tissue Location | Widely expressed; except in skeletal muscle where it is expressed at very low level (PubMed:15331600). Expressed in erythrocytes (PubMed:15137908). |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mammalian fructosamine-3-kinase (FN3K) catalyzes phosphorylation of fructosamines formed by glycation, the nonenzymatic reaction of glucose with primary amines. FN3K for example catalyzes transformation of fructoselysine (FL) residues on glycated proteins, to FL-3-phosphate (FL3P). This phosphorylation destablilizes FL and propels spontaneous decomposition, thereby reversing at an early stage the nonenzymatic glycation process. Phosphorylation of fructosamines may initiate metabolism of the modified amine and result in deglycation of glycated proteins. In mammals, FN3K has a closely related homologue, FN3KRP (FN3K-related protein), which in the human genome is located just 8 kb upstream of FN3K on chromosome 17q25.3 FN3KRP, which is 68% sequence identity with FN3K, is a deglycating enzyme which phosphorylates the third carbon of the sugar moiety of ketosamines, and may play a role in freeing proteins from ribulosamines or psicosamines. A deglycation system may be an important factor in protecting cells from the deleterious effects of nonenzymatic glycation, which appears to be an important factor in the pathogenesis of diabetic complications.
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