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ALPK1 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q96QP1 |
|---|---|
| Clone Names | 5071254 |
| Gene ID | 80216 |
|---|---|
| Other Names | Alpha-protein kinase 1, 2711-, Chromosome 4 kinase, Lymphocyte alpha-protein kinase, ALPK1, KIAA1527, LAK |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7109b was selected from the C-term region of human ALPK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | ALPK1 {ECO:0000303|PubMed:30111836, ECO:0000312|HGNC:HGNC:20917} |
|---|---|
| Function | Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity (PubMed:28877472, PubMed:28222186, PubMed:30111836). Specifically recognizes and binds ADP-D-glycero-beta- D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram- negative and some Gram-positive bacteria (PubMed:30111836). ADP- Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering pro-inflammatory NF-kappa-B signaling (PubMed:30111836). May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A (PubMed:27169898). May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A (PubMed:15883161). May play a role in ciliogenesis (PubMed:30967659). |
| Cellular Location | Cytoplasm, cytosol. Cytoplasm, cytoskeleton, spindle pole Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, cilium. Note=Localized at the base of primary cilia. |
| Tissue Location | Highly expressed in liver. Expressed in the optic nerve and retinal pigmented epithelium. Lower expression is observed in the macula and extramacular retina (PubMed:30967659) |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Unlike most eukaryotic kinases, alpha kinases, such as ALPK1, recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation. Epithelial cells maintain a polarized structure based on a selective sorting machinery for cargo traveling to the apical or the basolateral membrane domain at the trans-Golgi network exit. Alpha-kinase 1 (ALPK1)is a compnent of raft-carrying apical vesicles, originally identified in vesicles ferrying raft-associated sucrase-isomaltase (SI). It has been proposed that phosphorylation of myosin I by ALPK1 is essential to the apical trafficking of raft-associated SI.
References
Heine, M., et al., J. Biol. Chem. 280(27):25637-25643 (2005).Yamada, S., et al., Oncogene 23(35):5901-5911 (2004).Ryazanova, L.V., et al., Mol. Biol. (N.Y.) 35, 271-283 (2001) (): ().Ryazanov, A.G., et al., Curr. Biol. 9 (2), R43-R45 (1999) (): ().
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