|Other Names||Heat-stable enterotoxin receptor, STA receptor, hSTAR, Guanylyl cyclase C, GC-C, Intestinal guanylate cyclase, GUCY2C, GUC2C, STAR|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7136a was selected from the Center region of human GUCY2C. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptides guanylin and uroguanylin.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein Endoplasmic reticulum membrane; Single-pass type I membrane protein Note=The 145 kDa plasma membrane form of GC-C contains sialic acid and galactose residues, while a differencially glycosylated 130 Kda form is a high mannose form that is resident in the endoplasmic reticulum and may serve as the precursor for the cell surface form|
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Provided below are standard protocols that you may find useful for product applications.
Guanylyl cyclase C (GC-C) is a transmembrane receptor expressed primarily in the intestine that regulates chloride secretion via the cystic fibrosis transmembrane conductance regulator. Binding of GC-C to either the endogenous peptide guanylin or the bacterially derived heat-stable enterotoxin STa, results in increased levels of cGMP and the stimulation of water and chloride secretion. In the case of exposure to STa, this leads to debilitating secretory diarrhea. GUCY2C is a receptor for STa, and is also activated by the endogenous peptide guanylin. The GUCY2C receptor has an extracellular ligand-binding domain and a cytoplasmic guanylyl cyclase domain, as do members of the natriuretic peptide receptor family.
Singh, R., J. Biol. Chem. 278(27):24342-24349 (2003).Tien, Y.W., et al., Clin. Cancer Res. 9(13):4891-4896 (2003).Sindice, A., et al., J. Biol. Chem. 277(20):17758-17764 (2002).Scott, R.O., et al., J. Biol. Chem. 277(25):22934-22941 (2002).Park, J., et al., Cancer Epidemiol. Biomarkers Prev. 11(8):739-744 (2002).
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