|Other Names||Maternal embryonic leucine zipper kinase, hMELK, Protein kinase Eg3, pEg3 kinase, Protein kinase PK38, hPK38, Tyrosine-protein kinase MELK, MELK, KIAA0175|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7149a was selected from the Center region of human MELK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation and carcinogenesis. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14, possibly leading to affect mammary carcinogenesis by mediating inhibition of the pro-apoptotic function of BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis.|
|Cellular Location||Cell membrane; Peripheral membrane protein|
|Tissue Location||Expressed in placenta, kidney, thymus, testis, ovary and intestine.|
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Provided below are standard protocols that you may find useful for product applications.
MELK Contains 1 protein kinase domain that Belongs to the Ser/Thr protein kinase family. It phosphorylates ZNF622 and may contribute to its redirection to the nucleus. MELK may also be involved in the inhibition of spliceosome assembly during mitosis.
Davezac, N., et al., Oncogene 21(50):7630-7641 (2002).Heyer, B.S., et al., Mol. Reprod. Dev. 47(2):148-156 (1997).
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