|Other Names||MAP kinase-interacting serine/threonine-protein kinase 1, MAP kinase signal-integrating kinase 1, MAPK signal-integrating kinase 1, Mnk1, MKNK1, MNK1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7151a was selected from the N-term region of human MKNK1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||May play a role in the response to environmental stress and cytokines. Appears to regulate translation by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7- methylguanosine-containing mRNA cap.|
|Cellular Location||Isoform 2: Cytoplasm.|
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MKNK1 encodes a predicted 424-amino acid protein containing a sequence typical of the catalytic domain of serine/threonine kinases and a putative N-terminal nuclear localization signal sequence.C-terminal region of MKNK1 was phosphorylated and activated in vivo and in vitro by ERK1 and p38 MAP kinases, but not by JNK/SAPK. The activated ERK is essential for the activation of MKNK1 in insect cells. MKNK1 was activated following stimulation of HeLa cells with a variety of extracellular stimuli. MNK1 bind tightly to the growth factor-regulated MAP kinases, Erk1 and Erk2, and also binds strongly to the stress-activated kinase, p38.
Orton, K.C., et al., J. Biol. Chem. 279(37):38649-38657 (2004).Cuesta, R., et al., J. Virol. 78(14):7707-7716 (2004).Knauf, U., et al., Mol. Cell. Biol. 21(16):5500-5511 (2001).Cuesta, R., et al., Genes Dev. 14(12):1460-1470 (2000).Fukunaga, R., et al., EMBO J. 16(8):1921-1933 (1997).
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