PDXK Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | O00764 |
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Clone Names | 4012311 |
Gene ID | 8566 |
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Other Names | Pyridoxal kinase, Pyridoxine kinase, PDXK, C21orf124, C21orf97, PKH, PNK |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7167a was selected from the C-term region of human PDXK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | PDXK (HGNC:8819) |
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Function | Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (PubMed:9099727, PubMed:10987144, PubMed:17766369, PubMed:19351586, PubMed:31187503) (Probable). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions. |
Cellular Location | Cytoplasm, cytosol. |
Tissue Location | Ubiquitous (PubMed:9099727, PubMed:31187503). Highly expressed in testis (PubMed:9099727) |
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Provided below are standard protocols that you may find useful for product applications.
Background
Pyridoxal kinase (PDXK) converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The PDXK gene encodes a 312-amino acid polypeptide, and expression of the cDNA reveals pyridoxal kinase activity. Northern blot analysis revealed that a major 1.5-kb PDXK transcript is expressed in all tissues tested. The expression of PDXK shows circadian oscillations. The expression of Pdxk in mouse liver and brain is regulated by the 3 PAR bZIP transcription factors, Dbp, Hlf, and Tef, which also show circadian oscillations in expression. Mice devoid of all 3 transcription factors show decreased levels of brain PLP, serotonin, and dopamine, and are highly susceptible to frequently lethal generalized spontaneous and audiogenic epilepsies.
References
Shin, J.H., et al., Neurochem. Int. 45(1):73-79 (2004).Lee, H.S., et al., Mol. Cells 10(4):452-459 (2000).Laine-Cessac, P., et al., Biochem. Pharmacol. 54(8):863-870 (1997).Hanna, M.C., et al., J. Biol. Chem. 272(16):10756-10760 (1997).Zhang, Z., et al., J. Nutr. 10(1):53-59 (1993).
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