|Other Names||Pyridoxal kinase, Pyridoxine kinase, PDXK, C21orf124, C21orf97, PKH, PNK|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7167a was selected from the C-term region of human PDXK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Synonyms||C21orf124, C21orf97, PKH, PNK|
|Function||Required for synthesis of pyridoxal-5-phosphate from vitamin B6.|
|Tissue Location||Ubiquitous. Isoform 3 is detected in adult testis and spermatozoa|
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Provided below are standard protocols that you may find useful for product applications.
Pyridoxal kinase (PDXK) converts vitamin B6 to pyridoxal-5-phosphate (PLP), an essential cofactor in the intermediate metabolism of amino acids and neurotransmitters. The PDXK gene encodes a 312-amino acid polypeptide, and expression of the cDNA reveals pyridoxal kinase activity. Northern blot analysis revealed that a major 1.5-kb PDXK transcript is expressed in all tissues tested. The expression of PDXK shows circadian oscillations. The expression of Pdxk in mouse liver and brain is regulated by the 3 PAR bZIP transcription factors, Dbp, Hlf, and Tef, which also show circadian oscillations in expression. Mice devoid of all 3 transcription factors show decreased levels of brain PLP, serotonin, and dopamine, and are highly susceptible to frequently lethal generalized spontaneous and audiogenic epilepsies.
Shin, J.H., et al., Neurochem. Int. 45(1):73-79 (2004).Lee, H.S., et al., Mol. Cells 10(4):452-459 (2000).Laine-Cessac, P., et al., Biochem. Pharmacol. 54(8):863-870 (1997).Hanna, M.C., et al., J. Biol. Chem. 272(16):10756-10760 (1997).Zhang, Z., et al., J. Nutr. 10(1):53-59 (1993).
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