|Other Names||Riboflavin kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase, RFK|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7183a was selected from the N-term region of human RFK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.|
|Tissue Location||Detected in brain, placenta and urinary bladder|
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Provided below are standard protocols that you may find useful for product applications.
RFK (riboflavin kinase) catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).
Karthikeyan, S., et al., Biochemistry 42(43):12532-12538 (2003).Karthikeyan, S., et al., Structure (Camb.) 11(3):265-273 (2003).
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