RFK Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q969G6 |
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Clone Names | 4022301 |
Gene ID | 55312 |
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Other Names | Riboflavin kinase, ATP:riboflavin 5'-phosphotransferase, Flavokinase, RFK |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7183a was selected from the N-term region of human RFK. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | RFK |
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Function | Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF- activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. |
Cellular Location | Cytoplasm. |
Tissue Location | Detected in brain, placenta and urinary bladder. |
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Provided below are standard protocols that you may find useful for product applications.
Background
RFK (riboflavin kinase) catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN).
References
Karthikeyan, S., et al., Biochemistry 42(43):12532-12538 (2003).Karthikeyan, S., et al., Structure (Camb.) 11(3):265-273 (2003).
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