|Other Names||Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase, Myt1 kinase, PKMYT1, MYT1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7196b was selected from the c-terminal region of human PKMYT1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.|
|Cellular Location||Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein|
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The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Transcript Variant: This variant (1) encodes the longer isoform (1).
Dai, X., et al., J. Invest. Dermatol. 122(6):1356-1364 (2004).Nakajima, H., et al., J. Biol. Chem. 278(28):25277-25280 (2003).Passer, B.J., et al., Proc. Natl. Acad. Sci. U.S.A. 100(5):2284-2289 (2003).Okumura, E., et al., Nat. Cell Biol. 4(2):111-116 (2002).Booher, R.N., et al., J. Biol. Chem. 272(35):22300-22306 (1997).
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