|Other Names||Mitogen-activated protein kinase kinase kinase 12, Dual leucine zipper bearing kinase, DLK, Leucine-zipper protein kinase, ZPK, MAPK-upstream kinase, MUK, Mixed lineage kinase, MAP3K12, ZPK|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7214a was selected from the C-term region of human ZPK . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.|
|Cellular Location||Cytoplasm. Membrane; Peripheral membrane protein|
|Tissue Location||Highly expressed in brain and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
ZPK, a member of the Ser/Thr protein kinase family (MAP kinase kinase kinase subfamily), may be an activator of the JNK/SAPK pathway. It phosphorylates beta-casein, histone 1 and myelin basic protein in vitro, and interacts with MBIP through the leucine-zipper motif. ZPK is highly expressed in brain and kidney. It is phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated.
Fukuyama, K., et al., J. Biol. Chem. 275(28):21247-21254 (2000).Reddy, U.R., et al., Biochem. Biophys. Res. Commun. 202(1):613-620 (1994).
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