ZPK Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q12852 |
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Clone Names | 2031102 |
Gene ID | 7786 |
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Other Names | Mitogen-activated protein kinase kinase kinase 12, Dual leucine zipper bearing kinase, DLK, Leucine-zipper protein kinase, ZPK, MAPK-upstream kinase, MUK, Mixed lineage kinase, MAP3K12, ZPK |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7214a was selected from the C-term region of human ZPK . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | MAP3K12 |
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Synonyms | ZPK |
Function | Part of a non-canonical MAPK signaling pathway (PubMed:28111074). Activated by APOE, enhances the AP-1-mediated transcription of APP, via a MAP kinase signal transduction pathway composed of MAP2K7 and MAPK1/ERK2 and MAPK3/ERK1 (PubMed:28111074). May be an activator of the JNK/SAPK pathway. |
Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q60700}. Cell membrane {ECO:0000250|UniProtKB:Q60700}. Note=Behaves essentially as an integral membrane protein. {ECO:0000250|UniProtKB:Q60700} |
Tissue Location | Highly expressed in brain and kidney. |
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Provided below are standard protocols that you may find useful for product applications.
Background
ZPK, a member of the Ser/Thr protein kinase family (MAP kinase kinase kinase subfamily), may be an activator of the JNK/SAPK pathway. It phosphorylates beta-casein, histone 1 and myelin basic protein in vitro, and interacts with MBIP through the leucine-zipper motif. ZPK is highly expressed in brain and kidney. It is phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated.
References
Fukuyama, K., et al., J. Biol. Chem. 275(28):21247-21254 (2000).Reddy, U.R., et al., Biochem. Biophys. Res. Commun. 202(1):613-620 (1994).
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