|Other Names||Death-associated protein kinase 2, DAP kinase 2, DAP-kinase-related protein 1, DRP-1, DAPK2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7218a was selected from the N-term region of humanPK2 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation.|
|Cellular Location||Cytoplasm. Cytoplasmic vesicle, autophagosome lumen|
|Tissue Location||Isoform 2 is expressed in embryonic stem cells (at protein level). Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle. It is expressed abundantly in cells differentiated toward granulocytes and low in undifferentiated, normal and leukemic hematopoietic cells and monocytes/macrophages|
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Provided below are standard protocols that you may find useful for product applications.
DAPK2 belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites.
Satoh, A., et al., Br. J. Cancer 86(11):1817-1823 (2002).Chan, M.W., et al., Clin. Cancer Res. 8(2):464-470 (2002).Wong, T.S., et al., Clin. Cancer Res. 8(2):433-437 (2002).Shani, G., et al., EMBO J. 20(5):1099-1113 (2001).Inbal, B., et al., Mol. Cell. Biol. 20(3):1044-1054 (2000).
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