|Other Names||Transcription factor BTF3, Nascent polypeptide-associated complex subunit beta, NAC-beta, RNA polymerase B transcription factor 3, BTF3, NACB|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7291b was selected from the C-term region of human BTF3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.|
|Cellular Location||Cytoplasm. Nucleus. Note=The heterodimer with NACA is cytoplasmic|
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Provided below are standard protocols that you may find useful for product applications.
Basic transcription factor 3 (BTF3)forms a stable complex with RNA polymerase IIB and is required for transcriptional initiation.
Green,C.D.,Mol. Cancer Res. 5 (11), 1191-1200 (2007)Kanno M., Gene 117:219-228(1992)Kusumawidjaja,G.,Cancer Biol. Ther. 6 (3), 367-376 (2007)
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