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POLE3 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9NRF9 |
|---|---|
| Clone Names | 80917151 |
| Gene ID | 54107 |
|---|---|
| Other Names | DNA polymerase epsilon subunit 3, Arsenic-transactivated protein, AsTP, Chromatin accessibility complex 17 kDa protein, CHRAC-17, HuCHRAC17, DNA polymerase II subunit 3, DNA polymerase epsilon subunit p17, POLE3, CHRAC17 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7316a was selected from the N-term region of human POLE3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | POLE3 |
|---|---|
| Synonyms | CHRAC17 |
| Function | Accessory component of the DNA polymerase epsilon complex (PubMed:10801849). Participates in DNA repair and in chromosomal DNA replication (By similarity). Forms a complex with CHRAC1 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome-remodeling activity of ISWI/SNF2H and ACF1 (PubMed:10801849). Does not enhance nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex (PubMed:14759371). |
| Cellular Location | Nucleus. |
| Tissue Location | Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. |
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Provided below are standard protocols that you may find useful for product applications.
Background
POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.
References
Bolognese,F., Imbriano,C. Nucleic Acids Res. 28 (19), 3830-3838 (2000)Li,Y., Pursell,Z.F. J. Biol. Chem. 275 (30), 23247-23252 (2000)
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