|Other Names||Histone-lysine N-methyltransferase SMYD3, SET and MYND domain-containing protein 3, Zinc finger MYND domain-containing protein 1, SMYD3, ZMYND1, ZNFN3A1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7344a was selected from the N-term region of human SMYD3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Histone methyltransferase. Specifically methylates 'Lys- 4' of histone H3, inducing di- and tri-methylation, but not monomethylation (PubMed:15235609, PubMed:22419068). Also methylates 'Lys-5' of histone H4 (PubMed:22419068). Plays an important role in transcriptional activation as a member of an RNA polymerase complex (PubMed:15235609). Binds DNA containing 5'- CCCTCC-3' or 5'-GAGGGG-3' sequences (PubMed:15235609).|
|Cellular Location||Cytoplasm. Nucleus. Note=Mainly cytoplasmic when cells are arrested at G0/G1. Accumulates in the nucleus at S phase and G2/M.|
|Tissue Location||Expressed in skeletal muscles and testis. Overexpressed in a majority of colorectal and hepatocellular carcinomas.|
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Provided below are standard protocols that you may find useful for product applications.
SMYD3 is a histone methyltransferase that plays a role in transcriptional regulation as a member of an RNA polymerase complex.
Zou,J.N., Wang,S.Z. Cancer Lett. 280 (1), 78-85 (2009)Wang,S.Z., Luo,X.G. BMB Rep 41 (4), 294-299 (2008)Wang,H., Liu,Y. Cancer Sci. 99 (4), 787-791 (2008)Barlesi,F., Giaccone,G. Int. J. Cancer 122 (6), 1441-1442 (2008)Hamamoto,R., Silva,F.P. Cancer Sci. 97 (2), 113-118 (2006)
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