|Other Names||Nuclear pore complex protein Nup133, 133 kDa nucleoporin, Nucleoporin Nup133, NUP133|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7374c was selected from the Center region of human NUP133. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Involved in poly(A)+ RNA transport.|
|Cellular Location||Nucleus, nuclear pore complex Chromosome, centromere, kinetochore Note=Located on both the cytoplasmic and nuclear sides of the nuclear pore (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755).|
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Provided below are standard protocols that you may find useful for product applications.
The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.
Boehmer,T., Mol. Cell 30 (6), 721-731 (2008)Boehmer,T., Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 63 (PT 9), 816-818 (2007)
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