|Other Names||Golgi phosphoprotein 3, Coat protein GPP34, Mitochondrial DNA absence factor, MIDAS, GOLPH3, GPP34|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7445a was selected from the N-term region of human GOLPH3. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.|
|Cellular Location||Golgi apparatus, Golgi stack membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion intermembrane space. Cell membrane. Endosome. Note=Phosphatidylinositol 4-phosphate-binding and oligomerization participate in the recruitment onto Golgi membranes.|
|Tissue Location||Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. This protein is a peripheral membrane protein of the Golgi stack and may have a regulatory role in Golgi trafficking.
Ota T., Suzuki Y., Nishikawa T.Nat. Genet. 36:40-45(2004) Bell A.W., Ward M.A.J. Biol. Chem. 276:5152-5165(2001)
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