|Other Names||Histidine--tRNA ligase, cytoplasmic, Histidyl-tRNA synthetase, HisRS, HARS, HRS|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7567a was selected from the N-term region of human HARS. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Cytoplasmic histidine--tRNA ligase (Probable). Plays a role in axon guidance.|
|Tissue Location||Brain, heart, liver and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. HARS is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. This enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The protein is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.
Levine,S.M., Arthritis Rheum. 56 (8), 2729-2739 (2007)Lu,Q., Proc. Natl. Acad. Sci. U.S.A. 100 (13), 7626-7631 (2003)
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