|Other Names||Tryptophan--tRNA ligase, mitochondrial, (Mt)TrpRS, Tryptophanyl-tRNA synthetase, TrpRS, WARS2|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7586b was selected from the C-term region of human WARS2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Mitochondrion matrix.|
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Provided below are standard protocols that you may find useful for product applications.
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2.
Guo,L.T.,Nucleic Acids Res. 35 (17), 5934-5943 (2007)Liu,J., (er) Nucleic Acids Res. 32 (2), 719-727 (2004)Jorgensen,R., J. Biol. Chem. 275 (22), 16820-16826 (2000)
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