|Other Names||Sestrin-1, p53-regulated protein PA26, SESN1, PA26, SEST1|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7650b was selected from the C-term region of human Sestrin-1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway (PubMed:25263562, PubMed:26449471). This stress-inducible metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity). May positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1 (PubMed:23274085). May have an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein (By similarity). Was originally reported to contribute to oxidative stress resistance by reducing PRDX1 (PubMed:15105503). However, this could not be confirmed (By similarity).|
|Cellular Location||Nucleus. Cytoplasm|
|Tissue Location||Widely expressed.|
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Provided below are standard protocols that you may find useful for product applications.
Sestrin-1 is involved in the reduction of peroxiredoxins. This protein may also be regulator of cellular growth.
Budanov,A.V., Science 304 (5670), 596-600 (2004)Peeters,H., Hum. Genet. 112 (5-6), 573-580 (2003)Velasco-Miguel,S., Oncogene 18 (1), 127-137 (1999)
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