|Other Names||Carboxypeptidase E, CPE, Carboxypeptidase H, CPH, Enkephalin convertase, Prohormone-processing carboxypeptidase, CPE|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7657b was selected from the C-term region of human CPE. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Removes residual C-terminal Arg or Lys remaining after initial endoprotease cleavage during prohormone processing. Processes proinsulin.|
|Cellular Location||Isoform 1: Cytoplasmic vesicle, secretory vesicle membrane; Peripheral membrane protein. Secreted. Note=Associated with the secretory granule membrane through direct binding to lipid rafts in intragranular conditions.|
|Tissue Location||Isoform 2, but not isoform 1, is overexpressed in hepatocellular carcinoma (at protein level), as well as in other tumors, including pheochromocytomas and paragangliomas|
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Provided below are standard protocols that you may find useful for product applications.
CPE is a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in CPE gene are implicated in type II diabetes.
Oiso,S., J. Neurochem. 109 (1), 158-167 (2009)Yang,L., Ann. N. Y. Acad. Sci. 1150, 263-266 (2008)Jeffrey,K.D., Proc. Natl. Acad. Sci. U.S.A. 105 (24), 8452-8457 (2008)
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