|Other Names||Interferon-inducible double-stranded RNA-dependent protein kinase activator A, PKR-associated protein X, PKR-associating protein X, Protein activator of the interferon-induced protein kinase, Protein kinase, interferon-inducible double-stranded RNA-dependent activator, PRKRA, PACT, RAX|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7744a was selected from the S18 region of human PRKRA. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post- transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner (By similarity).|
|Cellular Location||Cytoplasm, perinuclear region. Cytoplasm.|
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Appears to have a pro-apoptotic function that may be suppressed in the presence of growth factor. Activates EIF2AK2 in absence of double stranded RNA (dsRNA).
Seibler,P., Lancet Neurol 7 (5), 380-381 (2008)Kok,K.H., J. Biol. Chem. 282 (24), 17649-17657 (2007)Patel,R.C., EMBO J. 17 (15), 4379-4390 (1998)
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