|Other Names||A-kinase anchor protein 7 isoforms alpha and beta, AKAP-7 isoforms alpha and beta, A-kinase anchor protein 18 kDa, AKAP 18, Protein kinase A-anchoring protein 7 isoforms alpha/beta, PRKA7 isoforms alpha/beta, AKAP7, AKAP15, AKAP18|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7750b was selected from the C-term region of human AKAP7. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium.|
|Cellular Location||Isoform Alpha: Lateral cell membrane; Lipid- anchor. Note=Targeted predominantly to the lateral membrane|
|Tissue Location||Expressed in brain, heart, lung, pancreas and skeletal muscle. EMBL; AF047715; AAC39715.1; -; mRNA EMBL; AF161075; AAF26676.1; -; mRNA EMBL; AK290271; BAF82960.1; -; mRNA EMBL; AL136110; CAI21507.1; -; Genomic_DNA EMBL; AL137063; CAI21507.1; JOINED; Genomic_DNA EMBL; AL136110; CAI21508.1; -; Genomic_DNA EMBL; AL137063; CAI21508.1; JOINED; Genomic_DNA EMBL; AL137063; CAI19001.1; -; Genomic_DNA EMBL; AL136110; CAI19001.1; JOINED; Genomic_DNA EMBL; AL137063; CAI19002.1; -; Genomic_DNA EMBL; AL136110; CAI19002.1; JOINED; Genomic_DNA EMBL; CH471051; EAW48057.1; -; Genomic_DNA EMBL; BC016927; AAH16927.1; -; mRNA EMBL; BC073847; AAH73847.1; -; mRNA EMBL; BC094732; AAH94732.1; -; mRNA CCDS; CCDS5143.1; -. [O43687-2] CCDS; CCDS5144.1; -. [O43687-1] RefSeq; NP_004833.1; NM_004842.3. [O43687-2] RefSeq; NP_619539.1; NM_138633.2. [O43687-1] UniGene; Hs.486483; - UniGene; Hs.732515; - ProteinModelPortal; O43687; - SMR; O43687; - BioGrid; 114851; 17 IntAct; O43687; 6 MINT; MINT-5000796; - STRING; 9606.ENSP00000405252; - iPTMnet; O43687; - SwissPalm; O43687; - BioMuta; EPB41L2; - PaxDb; O43687; - PeptideAtlas; O43687; - PRIDE; O43687; - DNASU; 9465; - Ensembl; ENST00000342266; ENSP00000345149; ENSG00000118507. [O43687-2] Ensembl; ENST00000474850; ENSP00000418208; ENSG00000118507. [O43687-1] GeneID; 9465; - UCSC; uc003qcm.3; human. [O43687-1] CTD; 9465; - DisGeNET; 9465; - GeneCards; AKAP7; - HGNC; HGNC:377; AKAP7 MIM; 604693; gene neXtProt; NX_O43687; - OpenTargets; ENSG00000118507; - PharmGKB; PA24671; - eggNOG; KOG2814; Eukaryota eggNOG; ENOG4111FMP; LUCA GeneTree; ENSGT00390000012756; - HOGENOM; HOG000013043; - HOVERGEN; HBG050474; - ChiTaRS; AKAP7; human GeneWiki; AKAP7; - GenomeRNAi; 9465; - Proteomes; UP000005640; Chromosome 6 Bgee; ENSG00000118507; - CleanEx; HS_AKAP7; - ExpressionAtlas; O43687; baseline and differential Genevisible; O43687; HS GO; GO:0016324; C:apical plasma membrane; IDA:UniProtKB GO; GO:0005622; C:intracellular; IEA:GOC GO; GO:0016328; C:lateral plasma membrane; IDA:UniProtKB GO; GO:0005886; C:plasma membrane; TAS:UniProtKB GO; GO:0051018; F:protein kinase A binding; IDA:UniProtKB GO; GO:0001508; P:action potential; IC:BHF-UCL GO; GO:0071320; P:cellular response to cAMP; IDA:BHF-UCL GO; GO:0035556; P:intracellular signal transduction; TAS:UniProtKB GO; GO:0006811; P:ion transport; TAS:ProtInc GO; GO:1902261; P:positive regulation of delayed rectifier potassium channel activity; IDA:BHF-UCL GO; GO:1901381; P:positive regulation of potassium ion transmembrane transport; IDA:BHF-UCL GO; GO:0008104; P:protein localization; IC:UniProtKB GO; GO:0060306; P:regulation of membrane repolarization; IDA:BHF-UCL InterPro; IPR019511; AKAP7_RI-RII-bd_dom Pfam; PF10470; AKAP7_RIRII_bdg; 1 1: Evidence at protein level; Alternative splicing; Cell membrane; Complete proteome; Lipoprotein; Membrane; Myristate; Palmitate; Reference proteome INIT_MET 1 1 Removed CHAIN 2 104 A-kinase anchor protein 7 isoforms alpha and beta /FTId=PRO_0000064522 REGION 2 11 Required for membrane localization REGION 18 40 Required for apical membrane localization REGION 52 65 RII-binding LIPID 2 2 N-myristoyl glycine LIPID 5 5 S-palmitoyl cysteine LIPID 6 6 S-palmitoyl cysteine VAR_SEQ 18 40 Missing (in isoform Alpha) /FTId=VSP_004101 MUTAGEN 2 6 GQLCC->AQLAA: Abolishes membrane localization and affects its ability to inhibit ENaC activity in a sodium- dependent manner SEQUENCE 104 AA; 11465 MW; 6F84ED8E032AF954 CRC64; MGQLCCFPFS RDEGKISELE SSSSAVLQRY SKDIPSWSSG EKNGGEPDDA ELVRLSKRLV ENAVLKAVQQ YLEETQNKNK PGEGSSVKTE AADQNGNDNE NNRK|
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Provided below are standard protocols that you may find useful for product applications.
AKAP7 is a member of the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAPs have a common RII-binding domain, but contain different targeting motifs responsible for directing PKA to distinct intracellular locations.
Brown,R.L., Biochem. Biophys. Res. Commun. 306 (2), 394-401 (2003)
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