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RAF1 Antibody (N-term) Blocking PeptideSynthetic peptide
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| BP7816a | 0.1 mg 400 ul | In Stock | $ 45.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
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RAF1 Antibody (N-term) Blocking Peptide - Product info | |
| Primary Accession | P04049 |
| Clone Names | 3020712 |
| Calculated MW | 73052 Da |
RAF1 Antibody (N-term) Blocking Peptide - Additional info | |
| Gene ID 5894 | |
| Target/Specificity The synthetic peptide sequence used to generate the antibody AP7816a was selected from the N-term region of human RAF1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. | |
| Format The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. | |
| Precautions This product is for research use only. Not for use in diagnostic or therapeutic procedures. | |
RAF1 Antibody (N-term) Blocking Peptide - Protein Information | |
| Name RAF1 | |
| Synonyms RAF | |
| Function Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions including proliferation, differentiation, apoptosis, survival and oncogenic transformation. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2). The phosphorylated form of RAF1 (on residues Ser-338 and Ser-339, by PAK1) phosphorylates BAD/Bcl2- antagonist of cell death at 'Ser-75'. Phosphorylates adenylyl cyclases: ADCY2, ADCY5 and ADCY6, resulting in their activation Phosphorylates PPP1R12A resulting in inhibition of the phosphatase activity. Phosphorylates TNNT2/cardiac muscle troponin T. Can promote NF-kB activation and inhibit signal transducers involved in motility (ROCK2), apoptosis (MAP3K5/ASK1 and STK3/MST2), proliferation and angiogenesis (RB1). Can protect cells from apoptosis also by translocating to the mitochondria where it binds BCL2 and displaces BAD/Bcl2-antagonist of cell death. Regulates Rho signaling and migration, and is required for normal wound healing. Plays a role in the oncogenic transformation of epithelial cells via repression of the TJ protein, occludin (OCLN) by inducing the up-regulation of a transcriptional repressor SNAI2/SLUG, which induces down-regulation of OCLN. Restricts caspase activation in response to selected stimuli, notably Fas stimulation, pathogen-mediated macrophage apoptosis, and erythroid differentiation | |
| Cellular Location Cytoplasm. Cell membrane. Mitochondrion. Nucleus. Note=Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. Phosphorylation at Ser-259 impairs its membrane accumulation. Recruited to the cell membrane by the active Ras protein. Phosphorylation at Ser-338 and Ser-339 by PAK1 is required for its mitochondrial localization. Retinoic acid- induced Ser-621 phosphorylated form of RAF1 is predominantly localized at the nucleus | |
| Tissue Location In skeletal muscle, isoform 1 is more abundant than isoform 2 | |
RAF1 Antibody (N-term) Blocking Peptide - Related products
AP3291a: Phospho-RAF1-S296 Antibody
AP3332a: Phospho-RAF1-S43 Antibody
AP3335a: Phospho-RAF1-S471 Antibody
AP3346a: Phospho-RAF1-T269 Antibody
AP3350a: Phospho-RAF1-pS338 Antibody
AP3443a: Phospho-RAF1-S259 Antibody
AP3445a: Phospho-RAF1-Y340 Antibody
AP3460a: Phospho-RAF1-S494 Antibody
AP3475a: Phospho-RAF1-Y341 Antibody
AP3511a: Phospho-RAF1-pS339 Antibody
AP7816a: RAF1 Antibody (N-term)
LY11633a: RAF1 Over-expression Lysate
BP3291a: Phospho-RAF1-S296 Antibody Blocking Peptide
BP3332a: Phospho-RAF1-S43 Antibody Blocking Peptide
BP3335a: Phospho-RAF1-S471 Antibody Blocking Peptide
BP3346a: Phospho-RAF1-T269 Antibody Blocking Peptide
BP3443a: Phospho-RAF1-S259 Antibody Blocking Peptide
BP3445a: Phospho-RAF1-Y340 Antibody Blocking Peptide
BP3460a: Phospho-RAF1-S494 Antibody Blocking Peptide
BP3475a: Phospho-RAF1-Y341 Antibody Blocking Peptide
BP3511a: Phospho-RAF1-pS339 Antibody Blocking Peptide
BP7816a: RAF1 Antibody (N-term) Blocking Peptide
BP7816d: RAF1 Antibody (S621) Blocking Peptide
BP7816e: RAF1 Antibody (S233) Blocking Peptide
BP7816f: RAF1 Antibody (S296) Blocking Peptide
BP7816g: RAF1 Antibody (S338) Blocking Peptide
BP7816h: RAF1 Antibody (Y340) Blocking Peptide
BP7816i: RAF1 Antibody (S471) Blocking Peptide
BP7816j: RAF1 Antibody (T269) Blocking Peptide
RAF1 Antibody (N-term) Blocking Peptide - Research Areas
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BACKGROUND
RAF1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, RAF1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
REFERENCES
Chong, H., et al., J. Biol. Chem. 278(38):36269-36276 (2003).Lee, M., et al., Cancer Lett. 193(1):57-64 (2003).Alavi, A., et al., Science 301(5629):94-96 (2003).Fringer, J., et al., J. Biol. Chem. 278(23):20612-20617 (2003).Oehrl, W., et al., J. Biol. Chem. 278(20):17819-17826 (2003).
