|Other Names||Methionine--tRNA ligase, cytoplasmic, Methionyl-tRNA synthetase, MetRS, MARS|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7844b was selected from the C-term region of human MARS. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
methionyl-tRNA synthetase belongs to the class I family of tRNA synthetases. It catalyzes the chemical reaction:ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Kaminska,M., Biochemistry 40 (47), 14309-14316 (2001)Kang,J., J. Biol. Chem. 275 (41), 31682-31688 (2000)Ko,Y.G., J. Cell Biol. 149 (3), 567-574 (2000)Quevillon,S., J. Mol. Biol. 285 (1), 183-195 (1999)
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