MARS Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | P56192 |
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Clone Names | 71228128 |
Gene ID | 4141 |
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Other Names | Methionine--tRNA ligase, cytoplasmic, Methionyl-tRNA synthetase, MetRS, MARS |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7844b was selected from the C-term region of human MARS. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | MARS1 (HGNC:6898) |
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Synonyms | MARS |
Function | Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed:11714285). Plays a role in the synthesis of ribosomal RNA in the nucleolus (PubMed:10791971). |
Cellular Location | Cytoplasm, cytosol. Nucleus, nucleolus. Note=Localizes to the nucleolus in proliferative cells but disappears in quiescent cells |
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Provided below are standard protocols that you may find useful for product applications.
Background
methionyl-tRNA synthetase belongs to the class I family of tRNA synthetases. It catalyzes the chemical reaction:ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
References
Kaminska,M., Biochemistry 40 (47), 14309-14316 (2001)Kang,J., J. Biol. Chem. 275 (41), 31682-31688 (2000)Ko,Y.G., J. Cell Biol. 149 (3), 567-574 (2000)Quevillon,S., J. Mol. Biol. 285 (1), 183-195 (1999)
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