|Other Names||Isoleucine--tRNA ligase, cytoplasmic, Isoleucyl-tRNA synthetase, IRS, IleRS, IARS|
|Target/Specificity||The synthetic peptide sequence used to generate the antibody AP7845a was selected from the N-term region of human IARS. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
|Format||Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.|
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.|
|Precautions||This product is for research use only. Not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.|
|Cellular Location||Cytoplasm. Cytoplasm, cytosol|
|Tissue Location||Expressed in liver and muscle (at protein level).|
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Provided below are standard protocols that you may find useful for product applications.
Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis. It catalyzes the chemical reaction:ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).
Shiba K., Proc. Natl. Acad. Sci. U.S.A. 91:7435-7439(1994)Nichols R.C., Gene 155:299-304(1995)
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